REG4 Protein (His tag)

Catalog No. ABIN7320368

Product Details

Target: REG4 (Regenerating Islet-Derived Family, Member 4 (REG4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This REG4 protein is labelled with His tag.

Purpose: Recombinant Mouse REG4/RELP Protein (His Tag)(Active)

Sequence: Met 1-Thr 157

Characteristics: A DNA sequence encoding mouse REG4 (NP_080604.2) (Met 1-Thr 157) was expressed with the polyhistide tag at the C-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion and proliferation of HCT-116 human colorectal carcinoma cells (ATCC: CCL-247) under low serum conditions. When 5 x 10E4 cells/well are added to mouse REG4 coated plates (0.16 μg/ml and 100 μl/well), >70% cell will adhere after 60 minutes at 37°C.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: REG4 (Regenerating Islet-Derived Family, Member 4 (REG4))

Alternative Name: REG4/RELP (REG4 Products)

Synonyms: GISP Protein, REG-IV Protein, RELP Protein, 2010002L15Rik Protein, regenerating family member 4 Protein, regenerating islet-derived family, member 4 Protein, Reg4 Protein, REG4 Protein

Background:

Background: Regenerating islet-derived protein 4, also known as REG-like protein, REG4, GISP and RELP, a member of the regenerating gene family belonging to the calcium (C-type) dependent lectin superfamily, has been found to be involved in malignancy in several different organs including the stomach, colorectum, pancreas and prostate. It is highly expressed in the gastrointestinal tract and markedly up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and inflammatory bowel disease. Expression of the Reg4 in different cell types has been associated with regeneration, cell growth and cell survival, cell adhesion and resistance to apoptosis. REG4 protein overexpression is associated with an unfavorable response to preoperative chemoradiotherapy and may be used as a predictive biomarker clinically. REG4 may play an important role in the development and progression of colorectal cancer, as well as in intestinal morphogenesis and epithelium restitution.

Synonym: 2010002L15Rik,GISP,RELP

Molecular Weight: 17.5 kDa

NCBI Accession: NP_080604