SPINT1 Protein (His tag)

Catalog No. ABIN7197928

Product Details

Target: SPINT1 (serine Peptidase Inhibitor, Kunitz Type 1 (SPINT1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SPINT1 protein is labelled with His tag.

Purpose: Recombinant Human HAI-1/SPINT1 Protein (His Tag)(Active)

Sequence: Met 1-Val 433

Characteristics: A DNA sequence encoding the human SPINT1 isoform 2 (O43278-2) (Met 1-Val 433) was fused with a polyhistidine tag at the C-terminus.

Purity: > 96 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (R&D Systems, Catalog # ES002). IC50 value is < 2nM.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SPINT1 (serine Peptidase Inhibitor, Kunitz Type 1 (SPINT1))

Alternative Name: HAI-1/SPINT1 (SPINT1 Products)

Synonyms: HAI-1 Protein, HAI Protein, HAI1 Protein, MANSC2 Protein, cb376 Protein, sb:cb376 Protein, spint1l Protein, zgc:64075 Protein, serine peptidase inhibitor, Kunitz type 1 Protein, serine protease inhibitor, Kunitz type 1 Protein, serine peptidase inhibitor, Kunitz type 1 b Protein, serine peptidase inhibitor, Kunitz type 1 a Protein, SPINT1 Protein, Spint1 Protein, spint1b Protein, spint1a Protein

Background:

Background: Vascular non-inflammatory molecule 2, also known as glycosyl-phosphatidyl inositol-anchored protein GPI-80, Vanin-2, Protein FOAP-4 and VNN2, is a cell membrane protein which belongs to the CN hydrolase family and Vanin subfamily. VNN2 is widely expressed with higher expression in spleen and blood. VNN2 is a member of the vanin family of proteins which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. VNN2 is an amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. It is involved in the thymus homing of bone marrow cells. VNN2 plays a role in transendothelial migration of neutrophils and may regulate beta-2 integrin-mediated cell adhesion, migration and motility of neutrophil.

Synonym: HAI;HAI1;MANSC2;SPINT1;UNQ223/PRO256

Molecular Weight: 45.8 kDa