SPINT2 Protein (His tag)

Catalog No. ABIN7197930

Product Details

Target: SPINT2 (serine Peptidase Inhibitor, Kunitz Type, 2 (SPINT2))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This SPINT2 protein is labelled with His tag.

Purpose: Recombinant Mouse HAI-2/SPINT2 Protein (His Tag)

Sequence: Met 1-Lys 140

Characteristics: A DNA sequence encoding the extracellular domain (Met 1-Lys 140) of mouse SPINT2 (NP_001076017.1) precursor was expressed with a C-terminal polyhistidine tag.

Purity: > 90 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SPINT2 (serine Peptidase Inhibitor, Kunitz Type, 2 (SPINT2))

Alternative Name: HAI-2/SPINT2 (SPINT2 Products)

Synonyms: HAI-2 Protein, Pb Protein, DIAR3 Protein, HAI2 Protein, Kop Protein, PB Protein, AL024025 Protein, C76321 Protein, serine peptidase inhibitor, Kunitz type, 2 Protein, serine peptidase inhibitor, Kunitz type 2 Protein, serine protease inhibitor, Kunitz type 2 Protein, Spint2 Protein, SPINT2 Protein

Background:

Background: Choanal (CA) and gastrointestinal atresias (GA) are an important feature of syndromic congenital sodium diarrhea (sCSD), a disorder recently associated with mutations in the gene for serine protease inhibitor type 2 (SPINT2). The SPINT2 gene is epigenetically silenced or downregulated in human cancers, altering the balance of HGF activation/inhibition ratio, which contributes to cancer development and progression. SPINT2 is a tumor suppressor gene that inhibits proteases implicated in cancer progression, like HGFA, hepsin and matriptase. Loss of SPINT2 expression in tumors has been associated with gene promoter hypermethylation. SPINT2 (serine peptidase inhibitor Kunitz type 2), a proteolytic inhibitor of hepatocyte growth factor activator (HGFA), which has a significant role in the suppression of the HGF-MET pathway and malignant melanoma progression.

Synonym: AL024025,C76321,HAI-2

Molecular Weight: 14 kDa

NCBI Accession: NP_001076017