STK26/MST4 Protein (GST tag)

Catalog No. ABIN7317098

Product Details

Target: STK26/MST4 (MST4) (Serine/threonine-Protein Kinase MST4 (MST4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This STK26/MST4 protein is labelled with GST tag.

Purpose: Recombinant Human MST4 Protein (GST Tag)(Active)

Sequence: Met 1-Pro 416

Characteristics: A DNA sequence encoding the human MST4 isoform 1 (NP_057626.2) (Met 1-Pro 416) was expressed with the fused GST tag at N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 15 nmol/min/mg using MBP as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 25 % glycerol, 0.6 mM GSH, 0.5 mM PMSF, 0.5 mM EDTA, 2 mM DTT

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: STK26/MST4 (MST4) (Serine/threonine-Protein Kinase MST4 (MST4))

Alternative Name: MST4 (MST4 Products)

Synonyms: Mst4 Protein, MASK Protein, RGD1563568 Protein, serine/threonine kinase 26 Protein, Stk26 Protein, STK26 Protein

Background:

Background: MST4, also known as mammalian STE20-like protein kinase 4, is a novel member of the germinal center kinase subfamily of human Ste20-like kinases and is closely related to MST3. The 416 amino acid full-length MST4 contains a C-terminal regulatory domain and an N-terminal kinase domain, both of which are required for full activation of the kinase. MST4 is highly expressed in placenta, thymus, and peripheral blood leukocytes. MST4 specifically activates ERK but not JNK or p38 MAPK in transient transfected cells or in stable cell lines, and thus is biologically active in the activation of MEK/ERK pathway mediating cell growth and transformation. Further, MST4 kinase activity is stimulated significantly by epidermal growth factor receptor (EGFR) ligands, which are known to promote growth of certain cancer cells. Accordingly, MST4 have a potential role in signal transduction pathways involved in cancer progression. Three alternatively spliced isoform of MST4 have been isolated, and isoform 3 lacks an exon encoding kinase domain and may function as a dominant-negative regulator of the MST4 kinase.

Synonym: MASK,MST4

Molecular Weight: 73 kDa

NCBI Accession: NP_057626