SERPINA3 Protein (His tag)

Catalog No. ABIN7197948

Product Details

Target: SERPINA3 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SERPINA3 protein is labelled with His tag.

Purpose: Recombinant Human SerpinA3/AACT Protein (His Tag)(Active)

Sequence: Met 1-Ala 423

Characteristics: A DNA sequence encoding the human SERPINA3 (NP_001076.2) (Met 1-Ala 423) was expressed, with a C-terminal polyhistidine tag.

Purity: > 97 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog# 27114). The IC50 value is < 5 nM.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 25 mM HEPES, 0.15M NaCl, pH 7.8

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SERPINA3 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3))

Alternative Name: SerpinA3/AACT (SERPINA3 Products)

Synonyms: SERPINA3-1 Protein, Pi2 Protein, AACT Protein, ACT Protein, GIG25 Protein, A1AT Protein, PI Protein, alpha-1-PI Protein, AAT Protein, Pi Protein, Spi1 Protein, SERPINA3-5 Protein, serpina3 Protein, serpin A3-5 Protein, serpin family A member 3 Protein, alpha-1-antiproteinase F precursor Protein, serpin family A member 1 Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 L homeolog Protein, LOC396684 Protein, SERPINA3 Protein, LOC100008973 Protein, SERPINA1 Protein, Serpina1 Protein, SPIA5 Protein, serpina3.L Protein

Background:

Background: SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a plasma alpha globulin glycoprotein, and is a member of serpin superfamily of the serine protease inhibitors consisting of at least 35 members. SerpinA3 has been demonstrated to inhibit the activity of certain serine proteases, such as cathepsin G found in neutrophils, and chymases present in mast cells, by inducing a major conformational rearrangement, and thus protects some tissues from damage caused by proteolytic enzymes. This enzyme is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease, and mutations of this gene have been observed in patients with Parkinson disease and chronic obstructive pulmonary disease. In addition, ACT gene polymorphism has been implicated with Alzheimer's disease (AD), cerebral amyloid angiopathy (CAA), as well as stroke, since SerpinA3 is a major constituent of the plaques in AD and an inhibitor of amyloid beta peptide degradation.

Synonym: Alpha-1-Antichymotrypsin, ACT, Cell Growth-Inhibiting Gene 24/25 Protein, Serpin A3, SERPINA3, AACT,GIG24,GIG25,MGC88254,SERPINA3

Molecular Weight: 46.5 kDa

NCBI Accession: NP_001076