SERPINA3 Protein (His tag)

Catalog No. ABIN7534222

Product Details

Target: SERPINA3 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SERPINA3 protein is labelled with His tag.

Purpose: Active Recombinant Human Serpin A3/Alpha-1-antichymotrypsin Protein

Sequence: NSPLDEENLT QENQDRGTHV DLGLASANVD FAFSLYKQLV LKAPDKNVIF SPLSISTALA FLSLGAHNTT LTEILKGLKF NLTETSEAEI HQSFQHLLRT LNQSSDELQL SMGNAMFVKE QLSLLDRFTE DAKRLYGSEA FATDFQDSAA AKKLINDYVK NGTRGKITDL IKDLDSQTMM VLVNYIFFKA KWEMPFDPQD THQSRFYLSK KKWVMVPMMS LHHLTIPYFR DEELSCTVVE LKYTGNASAL FILPDQDKME EVEAMLLPET LKRWRDSLEF REIGELYLPK FSISRDYNLN DILLQLGIEE AFTSKADLSG ITGARNLAVS QVVHKAVLDV FEEGTEASAA TAVKITLLSA LVETRTIVRF NRPFLMIIVP TDTQNIFFMS KVTNPKQA

Specificity: Asn26-Ala423

Purity: > 97 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 value is <11.5 nM.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of 20 mM HEPES,150 mM NaCl, pH 8.0.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: SERPINA3 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3))

Alternative Name: Serpin A3/Alpha-1-antichymotrypsin (SERPINA3 Products)

Synonyms: SERPINA3-1 Protein, Pi2 Protein, AACT Protein, ACT Protein, GIG25 Protein, A1AT Protein, PI Protein, alpha-1-PI Protein, AAT Protein, Pi Protein, Spi1 Protein, SERPINA3-5 Protein, serpina3 Protein, serpin A3-5 Protein, serpin family A member 3 Protein, alpha-1-antiproteinase F precursor Protein, serpin family A member 1 Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 Protein, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 L homeolog Protein, LOC396684 Protein, SERPINA3 Protein, LOC100008973 Protein, SERPINA1 Protein, Serpina1 Protein, SPIA5 Protein, serpina3.L Protein

Background:

Description: SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a plasma alpha globulin glycoprotein, and is a member of serpin superfamily of the serine protease inhibitors. It is synthesized primarily in the liver and secreted as one of the most abundant serpins in plasma. SerpinA3 deficiency has been associated with liver disease, and mutations of this gene have been observed in patients with Parkinson disease and chronic obstructive pulmonary disease. Serpin A3 is a major constituent of the plaques associated with Alzheimer's disease and an inhibitor of amyloid beta peptide degradation.

Name: AACT, ACT, GIG24, GIG25,SERPINA3,ACT,GIG24,GIG25

Gene ID: 12

UniProt: P01011