Siglec E Protein (Fc Tag)
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- Target See all Siglec E (Siglece) products
- Siglec E (Siglece) (Sialic Acid Binding Ig-Like Lectin E (Siglece))
- Protein Type
- Recombinant
- Origin
- Mouse
- Source
- Human Cells
- Purification tag / Conjugate
- This Siglec E protein is labelled with Fc Tag.
- Purpose
- Recombinant Mouse Siglec-E (C-Fc)
- Sequence
- Gln20-Phe355
- Characteristics
- Recombinant Mouse Sialic Acid Binding Ig-like Lectin E is produced by our Mammalian expression system and the target gene encoding Gln20-Phe355 is expressed with a Fc tag at the C-terminus.
- Purity
- >95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.5.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- Siglec E (Siglece) (Sialic Acid Binding Ig-Like Lectin E (Siglece))
- Alternative Name
- Siglec-E (Siglece Products)
- Synonyms
- Cd170 Protein, Siglec12 Protein, Siglec5 Protein, Siglec9 Protein, Siglecl1 Protein, mSiglec-E Protein, sialic acid binding Ig-like lectin E Protein, Siglece Protein
- Background
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Background: Siglecs are sialic acid specific I-type lectins that are characterized by an extracellular domain (ECD) with an N-terminal Ig-like V-set domain followed by varying numbers of Ig-like C2-set domains. Mouse Siglec-E, also known as Myeloid Inhibitory Siglec (MIS), is an 80 - 85 kDa member of the CD33-related subfamily of Siglecs. Rodent and primate Siglec gene families have significantly diverged, and Siglec-9 is the most likely human ortholog of mouse Siglec-E. Siglec-E is expressed as a heavily N-glycosylated disulfide-linked homodimer and shows binding preference for disialic acids in the alpha 2-8 linkage. Siglec-E is up-regulated and additionally phosphorylated following cellular stimulation by a variety of TLR agonists. Siglec-E signaling negatively regulates the LPS-induced production of TNF- alpha and IL-6 by macrophages. Its up-regulation in macrophages parallels the development of endotoxin tolerance. Siglec-E recognition of sialylated determinants on virulent T. cruzi contributes to the suppression of dendritic cell IL-12 p40 production.
Synonym: SiglecE, Siglec-E, Sialic Acid Binding Ig-like Lectin E
- Molecular Weight
- 64.4 kDa
- UniProt
- Q6PJ50
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