SLAMF7 Protein (His tag)

Catalog No. ABIN7320369

Product Details

Target: SLAMF7 (SLAM Family Member 7 (SLAMF7))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This SLAMF7 protein is labelled with His tag.

Purpose: Recombinant Mouse SLAMF7/CD319 Protein (His Tag)(Active)

Sequence: Met 1-Gly 224

Characteristics: A DNA sequence encoding the mouse SLAMF7 (NP_653122.2) extracellular domain (Met 1-Gly 224) was fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to bind biotinylated mouse SLAMF7 in a functional ELISA.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SLAMF7 (SLAM Family Member 7 (SLAMF7))

Alternative Name: SLAMF7/CD319 (SLAMF7 Products)

Synonyms: RGD1560190 Protein, SLAMF7 Protein, slaf7 Protein, 19A Protein, CD319 Protein, CRACC Protein, CS1 Protein, 19A24 Protein, 4930560D03Rik Protein, SLAM family member 7 Protein, Slamf7 Protein, SLAMF7 Protein, slaf7 Protein

Background:

Background: SLAM family member 7 (SLAMF7), also known as CRACC, CD319, CD2-like receptor-activating cytotoxic cells, and CS1, is a single-pass type I membrane protein and a member of the CD2 family of cell surface receptors. SLAMF7 is expressed in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-cell lines, or T-cell lines. Although the cytoplasmic domain of CS1 contains immunoreceptor tyrosine-based switch motifs (ITSM), which enables to recruite signaling lymphocyte activation molecule (SLAM)-associated protein (SAP/SH2D1A), it activates NK cells in the absence of a functional SAP. CS1 is a self ligand and homophilic interaction of CS1 regulates NK cell cytolytic activity. CRACC positively regulated natural killer cell functions by a mechanism dependent on the adaptor EAT-2 but not the related adaptor SAP. However, in the absence of EAT-2, CRACC potently inhibited natural killer cell function. It was also inhibitory in T cells, which are typically devoid of EAT-2. Thus, CRACC can exert activating or inhibitory influences on cells of the immune system depending on cellular context and the availability of effector proteins.

Synonym: SLAM family member 7, Leukocyte cell-surface antigen, Novel Ly9, CD319, Slamf7, CRACC,19A, 19A24, 4930560D03Rik, CRACCl, CS1

Molecular Weight: 23.7 kDa

NCBI Accession: NP_653122