TEK Protein (AA 770-1122) (His-GST)

Catalog No. ABIN7320054

Recombinant TEK protein expressed in Baculovirus infected Insect Cells.

Quick Overview for TEK Protein (AA 770-1122) (His-GST) (ABIN7320054)

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Baculovirus infected Insect Cells

Application: ELISA

Purity: > 91 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 770-1122

Purification tag / Conjugate: This TEK protein is labelled with His-GST.

Purpose: Recombinant Mouse Tie2/CD202b Protein (aa 770-1122, His & GST Tag)

Sequence: Gln770-Ala1122

Characteristics: A DNA sequence encoding the mouse TEK (Q02858) (Gln770-Ala1122) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. No Kinase Activity2. Immobilized mouse TEK (aa 770-1122) at 2 μg/ml (100 μl/well) can bind human Ang2-Fc with a linear range of 0.25-2.0 μg/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: Tie2/CD202b

Background: AA517024,Cd202b,Hyk,STK1,Tie-2,Tie2,TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.

Molecular Weight:

Calculated MW: 68.2 kDa

Observed MW: 68 kDa

Gene ID: 21687

UniProt: Q02858

Pathways: RTK Signaling, Growth Factor Binding