TRIB3 Protein (GST tag)

Catalog No. ABIN7198334

Product Details

Target: TRIB3 (Tribbles Homolog 3 (Drosophila) (TRIB3))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This TRIB3 protein is labelled with GST tag.

Purpose: Recombinant Human TRIB3/TRB3 Protein (GST Tag)

Sequence: Met 1-Gly 358

Characteristics: A DNA sequence encoding the human TRIB3 (NP_066981.2) (Met 1-Gly 358) was fused with the GST tag at N-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 0.5 mM Reduced Glutathione, 10 % glycerol, 0.5 mM PMSF

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: TRIB3 (Tribbles Homolog 3 (Drosophila) (TRIB3))

Alternative Name: TRIB3/TRB3 (TRIB3 Products)

Synonyms: zgc:76966 Protein, TRIB3 Protein, C20orf97 Protein, NIPK Protein, SINK Protein, SKIP3 Protein, TRB3 Protein, Ifld2 Protein, Nipk Protein, TRB-3 Protein, Trb3 Protein, tribbles pseudokinase 3 Protein, trib3 Protein, TRIB3 Protein, Trib3 Protein

Background:

Background: Tribbles homolog 3, also known as Neuronal cell death-inducible putative kinase, p65-interacting inhibitor of NF-kappa-B, SINK and TRIB3, is a Nucleus protein which belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family and Tribbles subfamily. Highest expression Of TRIB3 is in liver, pancreas, peripheral blood leukocytes and bone marrow. It is also highly expressed in a number of primary lung, colon and breast tumors. TRIB3 is expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney. TRIB3 disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. TRIB3 may bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. It binds to ATF4 and inhibits its transcriptional activation activity. TRIB3 interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. It interacts with MAPK kinases and regulates activation of MAP kinases. It may play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. TRIB3 does not display kinase activity.

Synonym: C20orf97;NIPK;SINK;SKIP3;TRB3

Molecular Weight: 65.8 kDa

NCBI Accession: NP_066981

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha