VAPB Protein (His tag)

Catalog No. ABIN7319355

Product Details

Target: VAPB (VAMP (Vesicle-Associated Membrane Protein)-Associated Protein B and C (VAPB))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This VAPB protein is labelled with His tag.

Purpose: Recombinant Human VAPB Protein (His Tag)(Active)

Sequence: Ala2-Pro132

Characteristics: Recombinant Human Vesicle-associated membrane protein-associated protein B is produced by our E.coli expression system and the target gene encoding Ala2-Pro132 is expressed with a 6His tag at the C-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human EphB2-Fc(Cat: PKSH032012) at 1μg/ml(100 μl/well) can bind Human VAMPB-His. The ED50 of Human VAMPB-His is 5.179ug/ml .

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: VAPB (VAMP (Vesicle-Associated Membrane Protein)-Associated Protein B and C (VAPB))

Alternative Name: VAPB (VAPB Products)

Synonyms: ALS8 Protein, VAMP-B Protein, VAP-B Protein, AI225786 Protein, AI840687 Protein, AI848259 Protein, D2Abb2e Protein, R75548 Protein, VAP33b Protein, Vamp33b Protein, fc05d09 Protein, wu:fa96d05 Protein, wu:fc05d09 Protein, zgc:66045 Protein, vamp-b Protein, vamp-c Protein, vap-b Protein, vap-c Protein, vapb Protein, VAMP associated protein B and C Protein, vesicle-associated membrane protein-associated protein B/C Protein, vesicle-associated membrane protein, associated protein B and C Protein, VAMP (vesicle-associated membrane protein)-associated protein B and C Protein, VAMP (vesicle-associated membrane protein)-associated protein B and C L homeolog Protein, VAPB Protein, Tsp_07972 Protein, Vapb Protein, vapb Protein, vapb.L Protein

Background:

Background: vesicle-associated membrane protein-associated protein B/C (VAPB) is presents in mitochondria-associated membranes that are endoplasmic reticulum membrane regions closely apposed to the outer mitochondrial membrane. It can form homodimer, and heterodimer with VAPA. It also interacts with VAMP1, VAMP2, HCV NS5A, NS5B, ZFYVE27 and RMDN3. VAPB participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. It is involved in cellular calcium homeostasis regulation.

Synonym: Vesicle-associated membrane protein-associated protein B/C,VAMP-B/VAMP-C,VAMP-associated protein B/C,VAP-B/VAP-C

Molecular Weight: 16.0 kDa

Pathways: ER-Nucleus Signaling