WWP2 Protein (GST tag,His tag)

Catalog No. ABIN7317302

Product Details

Target: WWP2 (WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This WWP2 protein is labelled with GST tag,His tag.

Purpose: Recombinant Human WWP2 Protein (His & GST Tag)

Sequence: Met 1-Glu870

Characteristics: A DNA sequence encoding the human WWP2 (O00308) (Met1-Glu870) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: WWP2 (WW Domain Containing E3 Ubiquitin Protein Ligase 2 (WWP2))

Alternative Name: WWP2 (WWP2 Products)

Synonyms: aip2 Protein, wwp2-like Protein, id:ibd1121 Protein, wu:fo87e10 Protein, zgc:154036 Protein, AIP2 Protein, WWp2-like Protein, 1300010O06Rik Protein, AA690238 Protein, AW554328 Protein, WW domain containing E3 ubiquitin protein ligase 2 Protein, WWP2 Protein, wwp2 Protein, Wwp2 Protein

Background:

Background: WWP2 contains 1 C2 domain, 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain and 4 WW domains. It is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. WWP2 can be detected in heart, throughout the brain, placenta, lung, liver, muscle, kidney and pancreas. It is also expressed in spleen and peripheral blood leukocytes. WWP2 polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation, in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. WWP2 ubiquitinates EGR2 and promotes it to proteasomal degradation, in T-cells the ubiquitination inhibits activation-induced cell death. It also ubiquitinates SLC11A2, the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. WWP2 ubiquitinates RPB1 and promotes it to proteasomal degradation.

Synonym: AIP2,WWp2-like

Molecular Weight: 126.7 kDa

UniProt: O00308

Pathways: Negative Regulation of Transporter Activity