HCoV-OC43 N Protein (His tag)
HCoV-OC43 N
Origin: Human Coronavirus OC43 (HCoV-OC43)
Host: Escherichia coli (E. coli)
Recombinant
> 90 % as determined by SDS-PAGE.
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- Target See all HCoV-OC43 N products
- HCoV-OC43 N (Coronavirus OC43 Nucleoprotein (HCoV-OC43 N))
- Protein Type
- Recombinant
- Origin
- Human Coronavirus OC43 (HCoV-OC43)
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This HCoV-OC43 N protein is labelled with His tag.
- Purpose
- Recombinant Human coronavirus (HCoV-OC43) Nucleoprotein / NP Protein (His Tag)
- Sequence
- Met1-Ile448
- Characteristics
- A DNA sequence encoding the Human coronavirus (HCoV-OC43) Nucleoprotein (YP_009555245.1) (Met1-Ile448) was expressed with a polyhistide tag at the N-terminus.
- Purity
- > 90 % as determined by SDS-PAGE.
- Endotoxin Level
- Please contact us for more information.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 40 mM PB, 500 mM NaCl, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- HCoV-OC43 N (Coronavirus OC43 Nucleoprotein (HCoV-OC43 N))
- Alternative Name
- Coronavirus OC-43 Nucleoprotein (HCoV-OC43 N Products)
- Background
- Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
- Molecular Weight
- 50.43KDa.
- NCBI Accession
- YP_009555245
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