Quick Overview for Leptin Protein (LEP) (His tag) (ABIN7321015)
Target
See all Leptin (LEP) ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This Leptin protein is labelled with His tag.
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Purpose
- Recombinant Carassiusauratus Leptin Protein (His Tag)
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Sequence
- Pro22-Cys171
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Characteristics
- Recombinant Carassiusauratus Leptin is produced by our Yeast expression system and the target gene encoding Pro22-Cys171 is expressed with a 8His tag at the N-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Leptin (LEP) (AA 22-167) protein (Fc Tag)
LEP Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leptin (LEP) proteinLEP Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by reducing SDS-PAGE.
Leptin (LEP) (AA 22-167) protein (Fc Tag)LEP Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Leptin (LEP) (AA 22-167) protein (His tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 22-167) protein (His tag)LEP Origin: Pig Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 22-167) protein (His-IF2DI Tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 22-167) protein (GST tag)LEP Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Leptin (LEP) (AA 428-635) protein (His-IF2DI Tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) proteinLEP Origin: Rat Host: Escherichia coli (E. coli) Recombinant 95,00% SDS
Leptin (LEP) (AA 29-164), (partial) protein (GST tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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Comment
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17 kDa
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please refer to the printed manual for detailed information.
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Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Leptin (LEP)
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Alternative Name
- Leptin
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Background
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Background: Leptin is a hormone secreted from white adipocytes and plays important role in the regulation of food intake and energy balance. Leptin functions via signaling pathways involving OB-R in hypothalamus. In mammals, leptin is mainly produced by the adipose tissue and encodes body fat reserves, acting as a short-term satiety signal. In fish, the presence of a leptin-like peptide was first evidenced by immuno-cross-reactivity, and its existence was certainly demonstrated after the finding by synteny of a leptin sequence in the pufferfish.
Synonym: Leptin, Obese Protein, Obesity Factor, LEP, OB, OBS
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Molecular Weight
- 18.3 kDa
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UniProt
- B8YI02
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Pathways
- JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
Target
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