HLA DRA*01:01-DRB1*04:01 protein (V5 tag,SBP Tag,His tag)

Catalog No. ABIN7273234

Product Details

Target: HLA DRA*01:01-DRB1*04:01

Protein Type: Recombinant

Origin: Human

Source: Mammalian Cells

Purification tag / Conjugate: V5 tag,SBP Tag,His tag

Application: Blocking Peptide (BP), Control Peptide (CP), ELISA, Neutralization (Neut)

Purpose: The product is HLA DRA*01:01-DRB1*04:01 as zipper-stabilized heterodimer

Sequence: alpha chain: IKEEHVIIQA EFYLNPDQSG EFMFDFDGDE IFHVDMAKKE TVWRLEEFGR FASFEAQGAL ANIAVDKANL EIMTKRSNYT PITNVPPEVT VLTNSPVELR EPNVLICFID KFTPPVVNVT WLRNGKPVTT GVSETVFLPR EDHLFRKFHY LPFLPSTEDV YDCRVEHWGL DEPLLKHWEF DAPSPLPETT E
beta chain: GDTRPRFLEQ VKHECHFFNG TERVRFLDRY FYHQEEYVRF DSDVGEYRAV TELGRPDAEY WNSQKDLLEQ KRAAVDTYCR HNYGVGESFT VQRRVYPEVT VYPAKTQPLQ HHNLLVCSVN GFYPGSIEVR WFRNGQEEKT GVVSTGLIQN GDWTFQTLVM LETVPRSGEV YTCQVEHPSL TSPLTVEWRA RSESAQSK
Sequences without tags. Tag location is at the discretion of the manufacturer.

Specificity: HLA DRA*01:01-DRB1*04:01, P01903 / P01911
HLA-DRB1*04:01 is associated with different diseases such as multiple sclerosis, rheumatoid arthritis or type 1 diabetes mellitus. People bearing HLA-DRB1*04:01 are more likely to be asymptomatic during an infection with SARS-CoV-2.

Characteristics: HLAs are produced in mammalian cell cultures as recombinant, truncated, soluble antigens, as zipper-stabilized heterodimers (HLA class II). As part of our quality control, all HLA are analyzed for purity by gel electrophoresis, concentration is determined by ELISA (0.5 mg/ml) and their functionality is verified with a specific antiserum.

Purification: Chromatography

Purity: > 80 %

Endotoxin Level: Endotoxins have not been removed

Application Details

Application Notes: ELISA: 10 - 1000 ng/mL

Comment:

sandwich ELISA, direct ELISA, bead ELISA, reactive with antibodies anti-V5 and anti-His and specific antiserum, B cell ELI Spot (predicted), in vitro memory B cell stimulation (predicted), HLA competition assay (predicted), control in various assays (predicted)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate based buffer, pH 7.8, Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 6 months

Target Details

Target: HLA DRA*01:01-DRB1*04:01

Background: Human Leukozyte Antigen, human Antigen-presenting major histocompatibility complex class II (MHCII) molecule. Expressed on Antigen-presenting cells (APC), displays peptides to CD4-positive T cells. Involved in cellular immune response.

Molecular Weight: approx. 65 - 70 kDa (approx. 30 kDa alpha chain and 35 - 39 kDa beta chain)

UniProt: P01903