HLA DPA1*02:02-DPB1*05:01 protein (V5 tag,SBP Tag,His tag)
Origin: Human
Host: Mammalian Cells
Recombinant
> 80 %
BP, CP, ELISA, Neut
-
- Target
- HLA DPA1*02:02-DPB1*05:01
- Protein Type
- Recombinant
- Origin
- Human
- Source
- Mammalian Cells
- Purification tag / Conjugate
- V5 tag,SBP Tag,His tag
- Application
- Blocking Peptide (BP), Control Peptide (CP), ELISA, Neutralization (Neut)
- Purpose
- The product is HLA DPA1*02:02-DPB1*05:01 as zipper-stabilized heterodimer
- Sequence
-
alpha chain: AGAIKADHVS TYAMFVQTHR PTGEFMFEFD EDEQFYVDLD KKETVWHLEE FGRAFSFEAQ GGLANIAILN NNLNTLIQRS NHTQAANDPP EVTVFPKEPV ELGQPNTLIC HIDRFFPPVL NVTWLCNGEP VTEGVAESLF LPRTDYSFHK FHYLTFVPSA EDVYDCRVEH WGLDQPLLKH WEAQEPIQ
beta chain: PENYLFQGRQ ECYAFNGTQR FLERYIYNRE ELVRFDSDVG EFRAVTELGR PEAEYWNSQK DILEEKRAVP DRMCRHNYEL DEAVTLQRRV QPKVNVSPSK KGPLQHHNLL VCHVTDFYPG SIQVRWFLNG QEETAGVVST NLIRNGDWTF QILVMLEMTP QQGDVYICQV EHTSLDSPVT VEWKAQSDSA RSKT
Sequences without tags. Tag location is at the discretion of the manufacturer. - Specificity
- HLA DPA1*02:02-DPB1*05:01, P20036 / P04440
- Characteristics
- HLAs are produced in mammalian cell cultures as recombinant, truncated, soluble antigens, as zipper-stabilized heterodimers (HLA class II). As part of our quality control, all HLA are analyzed for purity by gel electrophoresis, concentration is determined by ELISA (0.5 mg/ml) and their functionality is verified with a specific antiserum.
- Purification
- Chromatography
- Purity
- > 80 %
- Endotoxin Level
- Endotoxins have not been removed
-
- Application Notes
- ELISA: 10 - 1000 ng/mL
- Comment
-
sandwich ELISA, direct ELISA, bead ELISA, reactive with antibodies anti-V5 and anti-His and specific antiserum, B cell ELI Spot (predicted), in vitro memory B cell stimulation (predicted), HLA competition assay (predicted), control in various assays (predicted)
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Phosphate based buffer, pH 7.8, Sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Expiry Date
- 6 months
-
- Target
- HLA DPA1*02:02-DPB1*05:01
- Background
- Human Leukozyte Antigen, human Antigen-presenting major histocompatibility complex class II (MHCII) molecule. Expressed on Antigen-presenting cells (APC), displays peptides to CD4-positive T cells. Involved in cellular immune response.
- Molecular Weight
- approx. 60 - 65 kDa (approx. 28 kDa alpha chain and 33 - 37 kDa beta chain)
- UniProt
- P20036
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