HLA DQA1*01:03-DQB1*06:01 protein (V5 tag,SBP Tag,His tag)
Origin: Human
Host: Mammalian Cells
Recombinant
> 80 %
BP, CP, ELISA, Neut
-
- Target
- HLA DQA1*01:03-DQB1*06:01
- Protein Type
- Recombinant
- Origin
- Human
- Source
- Mammalian Cells
- Purification tag / Conjugate
- V5 tag,SBP Tag,His tag
- Application
- Blocking Peptide (BP), Control Peptide (CP), ELISA, Neutralization (Neut)
- Purpose
- The product is HLA DQA1*01:03-DQB1*06:01 as zipper-stabilized heterodimer
- Sequence
-
alpha chain: EDIVADHVAS YGVNLYQSYG PSGQFTHEFD GDEEFYVDLE RKETVWKLPL FHRLRFDPQF ALTNIAVLKH NLNILIKRSN STAATNEVPE VTVFSKSPVT LGQPNTLICL VDNIFPPVVN ITWLSNGHSV TEGVSETSFL SKSDHSFFKI SYLTFLPSAD EIYDCKVEHW GLDEPLLKHW EPEIPAPMSE LTET
beta chain: RDPPEDFVLQ FKAMCYFTNG TERVRYVTRY IYNREEDVRF DSDVGVYRAV TPQGRPDAEY WNSQKDILER TRAELDTVCR HNYEVAFRGI LQRRVEPTVT ISPSRTEALN HHNLLVCSVT DFYPGQIKVR WFRNDQEETA GVVSTPLIRN GDWTFQILVM LEMTPQHGDV YTCHVEHPSL QSPITVEWRA QSESAQNK
Sequences without tags. Tag location is at the discretion of the manufacturer. - Specificity
- HLA DQA1*01:03-DQB1*06:01, P01909 / P01920
- Characteristics
- HLAs are produced in mammalian cell cultures as recombinant, truncated, soluble antigens, as zipper-stabilized heterodimers (HLA class II). As part of our quality control, all HLA are analyzed for purity by gel electrophoresis, concentration is determined by ELISA (0.5 mg/ml) and their functionality is verified with a specific antiserum.
- Purification
- Chromatography
- Purity
- > 80 %
- Endotoxin Level
- Endotoxins have not been removed
-
- Application Notes
- ELISA: 10 - 1000 ng/mL
- Comment
-
sandwich ELISA, direct ELISA, bead ELISA, reactive with antibodies anti-V5 and anti-His and specific antiserum, B cell ELI Spot (predicted), in vitro memory B cell stimulation (predicted), HLA competition assay (predicted), control in various assays (predicted)
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Phosphate based buffer, pH 7.8, Sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Expiry Date
- 6 months
-
- Target
- HLA DQA1*01:03-DQB1*06:01
- Background
- Human Leukozyte Antigen, human Antigen-presenting major histocompatibility complex class II (MHCII) molecule. Expressed on Antigen-presenting cells (APC), displays peptides to CD4-positive T cells. Involved in cellular immune response.
- Molecular Weight
- approx. 60 - 65 kDa (approx. 29 kDa alpha chain and 34 - 38 kDa beta chain)
- UniProt
- P01909
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