ACE2 Protein (AA 18-740) (His tag)

Catalog No. ABIN7273791

Product Details

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Protein Type: Recombinant

Protein Characteristics: AA 18-740

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ACE2 protein is labelled with His tag.

Purpose: Human ACE2/ACEH Protein

Sequence: Gln18-Ser740

Characteristics: Recombinant Human ACE2/ACEH Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln18-Ser740.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 11.9nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ACE2 (Angiotensin I Converting Enzyme 2 (ACE2))

Alternative Name: ACE2 (ACE2 Products)

Synonyms: ACE1 Protein, CD143 Protein, DCP Protein, DCP1 Protein, ICH Protein, MVCD3 Protein, ACEH Protein, 2010305L05Rik Protein, zgc:92514 Protein, angiotensin I converting enzyme Protein, angiotensin I converting enzyme 2 Protein, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 Protein, ACE Protein, ACE2 Protein, Ace2 Protein, ace2 Protein

Background: ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension.The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.

Molecular Weight: 84.7 kDa. Due to glycosylation, the protein migrates to 85-110 kDa based on Tris-Bis PAGE result.

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour