AMIGO2 Protein (AA 39-397) (His tag)
-
- Target See all AMIGO2 Proteins
- AMIGO2 (Adhesion Molecule with Ig-Like Domain 2 (AMIGO2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 39-397
-
Origin
- Cynomolgus
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This AMIGO2 protein is labelled with His tag.
- Purpose
- Cynomolgus AMIGO2 Protein
- Sequence
- Val39-Thr397
- Characteristics
- Recombinant Cynomolgus AMIGO2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val39-Thr397.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Top Product
- Discover our top product AMIGO2 Protein
-
Adhesion Molecule with Ig-Like Domain 2 (AMIGO2) (AA 39-397) protein (His tag)
AMIGO2 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Adhesion Molecule with Ig-Like Domain 2 (AMIGO2) (AA 40-398) protein (His tag)AMIGO2 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Adhesion Molecule with Ig-Like Domain 2 (AMIGO2) (AA 1-522) protein (GST tag)AMIGO2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Adhesion Molecule with Ig-Like Domain 2 (AMIGO2) (AA 420-522) protein (His tag)Crystallography grade AMIGO2 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Adhesion Molecule with Ig-Like Domain 2 (AMIGO2) (AA 419-519) protein (His tag)Crystallography grade AMIGO2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Adhesion Molecule with Ig-Like Domain 2 (AMIGO2) (AA 39-397) protein (His tag)Crystallography grade AMIGO2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Adhesion Molecule with Ig-Like Domain 2 (AMIGO2) (AA 40-522) protein (rho-1D4 tag)Crystallography grade AMIGO2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Adhesion Molecule with Ig-Like Domain 2 (AMIGO2) (AA 39-519) protein (rho-1D4 tag)Crystallography grade AMIGO2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- AMIGO2 (Adhesion Molecule with Ig-Like Domain 2 (AMIGO2))
- Alternative Name
- AMIGO2 (AMIGO2 Products)
- Synonyms
- AMIGO2 Protein, AI415330 Protein, AMIGO-2 Protein, AW208913 Protein, Ali1 Protein, ALI1 Protein, DEGA Protein, adhesion molecule with Ig like domain 2 Protein, adhesion molecule with Ig-like domain 2 Protein, AMIGO2 Protein, Amigo2 Protein
- Background
- Bromodomain and extraterminal domain inhibitors (BETi) represent promising therapeutic agents for metastatic melanoma, yet their mechanism of action remains unclear. Here we interrogated the transcriptional effects of BETi and identified AMIGO2, a transmembrane molecule, as a BET target gene essential for melanoma cell survival. AMIGO2 is upregulated in melanoma cells and tissues compared to human melanocytes and nevi, and AMIGO2 silencing in melanoma cells induces G1/S arrest followed by apoptosis.
- Molecular Weight
- 41.97 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
- UniProt
- G7PHN7
-
