Apelin Protein (APLN) (AA 23-77) (Fc Tag)
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- Target See all Apelin (APLN) Proteins
- Apelin (APLN)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 23-77
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Apelin protein is labelled with Fc Tag.
- Purpose
- Human APLN Protein
- Sequence
- Gly23-Phe77
- Characteristics
- Recombinant Human APLN Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly23-Phe77.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human APLN, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-APLN Antibody, hFc Tag with the EC50 of 19.0ng/ml determined by ELISA. See testing image for detail.
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- Discover our top product APLN Protein
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Apelin (APLN) (AA 23-77) protein (Fc Tag)
APLN Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Apelin (APLN) (AA 23-77) protein (Fc-Avi Tag,Biotin)APLN Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Apelin (APLN) (AA 1-77) protein (GST tag)APLN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Apelin (APLN) (AA 1-122) protein (GST tag)APLN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS, 200 mM Arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Apelin (APLN)
- Alternative Name
- APLN (APLN Products)
- Synonyms
- APLN Protein, Xapelin Protein, xnpep2 Protein, preproapelin Protein, 6030430G11Rik Protein, Apel Protein, APEL Protein, XNPEP2 Protein, apelin Protein, apelin S homeolog Protein, apln Protein, APLN Protein, apln.S Protein, Apln Protein
- Background
- Macrophages play key roles during cardiovascular diseases (CVD) and their related complications.Apelin (APLN) is a key molecule, whose roles during CVD have been documented previously. Therefore, it has been hypothesized that APLN may perform its roles via modulation of macrophages. Additionally, due to the widespread distribution of the CVD, more effective therapeutic strategies need to be developed to overcome the related complications.
- Molecular Weight
- 32.9 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
- UniProt
- Q9ULZ1
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Feeding Behaviour
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