ICOSLG Protein (AA 19-258) (His-Avi Tag)

Catalog No. ABIN7273967

Product Details

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Protein Type: Recombinant

Protein Characteristics: AA 19-258

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ICOSLG protein is labelled with His-Avi Tag.

Purpose: Human B7-H2/ICOSLG Protein

Sequence: Asp19-Ser258

Characteristics: Recombinant Human B7-H2/ICOSLG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp19-Ser258.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human B7-H2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human ICOS, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA. The affinity constant of 25.59 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Alternative Name: B7-H2 (ICOSLG Products)

Synonyms: ICOSLG Protein, AU044799 Protein, B7-H2 Protein, B7RP-1 Protein, B7h Protein, BG071784 Protein, GI50 Protein, GL50 Protein, GL50-B Protein, ICOS-L Protein, Icoslg Protein, LICOS Protein, Ly115l Protein, mKIAA0653 Protein, B7H2 Protein, B7RP1 Protein, CD275 Protein, ICOSL Protein, Icosl Protein, RGD1562791 Protein, inducible T-cell costimulator ligand Protein, icos ligand Protein, inducible T-cell co-stimulator ligand Protein, ICOSLG Protein, Icosl Protein, Icoslg Protein

Background: B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion, induces also B-cell proliferation and differentiation into plasma cells.

Molecular Weight: 29.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

UniProt: O75144

Pathways: Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation