BST2 Protein (AA 49-161) (Fc Tag)

Catalog No. ABIN7273993

Product Details

Target: BST2 (Bone Marrow Stromal Cell Antigen 2 (BST2))

Protein Type: Recombinant

Protein Characteristics: AA 49-161

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This BST2 protein is labelled with Fc Tag.

Purpose: Human BST2 Protein

Sequence: Asn49-Ser161

Characteristics: Recombinant Human BST2 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Asn49-Ser161.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human BST2, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-BST2 Antibody, hFc Tag with the EC50 of 60.4ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: BST2 (Bone Marrow Stromal Cell Antigen 2 (BST2))

Alternative Name: BST2 (BST2 Products)

Synonyms: BST2 Protein, CD317 Protein, TETHERIN Protein, 2310015I10Rik Protein, Bst-2 Protein, C87040 Protein, DAMP-1 Protein, GREG Protein, Damp1 Protein, bone marrow stromal cell antigen 2 Protein, bone marrow stromal antigen 2 Protein, BST2 Protein, LOC618737 Protein, Bst2 Protein

Background: Interferon-induced BST2 (bone marrow stromal cell antigen 2) inhibits viral replication by tethering enveloped virions to the cell surface to restrict viral release and by inducing the NFKB-dependent antiviral immune response. BST2 expression was significantly increased during porcine epidemic diarrhea virus (PEDV) infection of Vero cells by IRF1 targeting its promoter. Both the BST2 and N protein interacted with the E3 ubiquitin ligase MARCHF8/MARCH8 and the cargo receptor.

Molecular Weight: 40 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Pathways: Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response