BTN3A1 Protein (AA 1-219) (His-Avi Tag,Biotin)
-
- Target See all BTN3A1 Proteins
- BTN3A1 (Butyrophilin, Subfamily 3, Member A1 (BTN3A1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-219
-
Origin
- Cynomolgus
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This BTN3A1 protein is labelled with His-Avi Tag,Biotin.
- Purpose
- Biotinylated Cynomolgus BTN3A1/CD277 Protein
- Sequence
- Gln1-Trp219
- Characteristics
- Recombinant Biotinylated Cynomolgus BTN3A1/CD277 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln1-Trp219.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-BTN3A1 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus BTN3A1, His Tag with the EC50 of 5.4ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product BTN3A1 Protein
-
Butyrophilin, Subfamily 3, Member A1 (BTN3A1) (AA 30-254) protein (His tag)
BTN3A1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Butyrophilin, Subfamily 3, Member A1 (BTN3A1) (AA 30-254) protein (Fc Tag)BTN3A1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Butyrophilin, Subfamily 3, Member A1 (BTN3A1) (AA 1-219) protein (His-Avi Tag)BTN3A1 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Butyrophilin, Subfamily 3, Member A1 (BTN3A1) (AA 30-254) protein (His-Avi Tag,Biotin)BTN3A1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Butyrophilin, Subfamily 3, Member A1 (BTN3A1) protein (mFc-His Tag)BTN3A1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Butyrophilin, Subfamily 3, Member A1 (BTN3A1) protein (Biotin)BTN3A1 Origin: Human Host: Human Cells Recombinant >90 % as determined by reducing SDS-PAGE.
Butyrophilin, Subfamily 3, Member A1 (BTN3A1) (AA 272-513) protein (His tag)Crystallography grade BTN3A1 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Butyrophilin, Subfamily 3, Member A1 (BTN3A1) (AA 30-254) protein (His tag)Crystallography grade BTN3A1 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Butyrophilin, Subfamily 3, Member A1 (BTN3A1) (AA 30-513) protein (rho-1D4 tag)Crystallography grade BTN3A1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- BTN3A1 (Butyrophilin, Subfamily 3, Member A1 (BTN3A1))
- Alternative Name
- BTN3A1 (BTN3A1 Products)
- Synonyms
- BT3.1 Protein, BTF5 Protein, CD277 Protein, BTN3A1 Protein, butyrophilin subfamily 3 member A1 Protein, BTN3A1 Protein, LOC100447925 Protein
- Background
- The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).
- Molecular Weight
- 26.5 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.
- UniProt
- A0A330KWZ1
- Pathways
- Activated T Cell Proliferation
-
