CD34 Protein (CD34) (AA 32-290) (His tag)
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- Target See all CD34 Proteins
- CD34
- Protein Type
- Recombinant
- Protein Characteristics
- AA 32-290
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD34 protein is labelled with His tag.
- Purpose
- Human CD34 Protein
- Sequence
- Ser32-Thr290
- Characteristics
- Recombinant Human CD34 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser32-Thr290.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD34, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD34 Antibody, hFc Tag with the EC50 of 16.5ng/ml determined by ELISA. See testing image for detail.
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CD34 (CD34) (AA 35-287) protein (His tag)
CD34 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD34 (CD34) (AA 124-225) protein (His tag)CD34 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
CD34 (CD34) (AA 1-328) protein (GST tag)CD34 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
CD34 (CD34) (AA 32-141) protein (GST tag)CD34 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
CD34 (CD34) protein (Fc Tag)CD34 Origin: Rat Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE
CD34 (CD34) (Active) protein (His tag)CD34 Origin: Mouse Host: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
CD34 (CD34) (AA 32-290) protein (His tag)Crystallography grade CD34 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
CD34 (CD34) (AA 35-287) protein (His tag)Crystallography grade CD34 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
CD34 (CD34) (AA 32-385) protein (rho-1D4 tag)Crystallography grade CD34 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
CD34 (CD34) (AA 35-382) protein (rho-1D4 tag)Crystallography grade CD34 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD34
- Alternative Name
- CD34 (CD34 Products)
- Synonyms
- AU040960 Protein, CD34 molecule Protein, CD34 antigen Protein, CD34 Protein, Cd34 Protein
- Background
- CD34 is a transmembrane phosphoglycoprotein, first identified on hematopoietic stem and progenitor cells. Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. Due to these historical and clinical associations, CD34 expression is almost ubiquitously related to hematopoietic cells, and it is a common misconception that CD34-positive (CD34()) cells in nonhematopoietic samples represent hematopoietic contamination.
- Molecular Weight
- 28.6 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Tris-Bis PAGE result.
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