CD73 Protein (AA 29-549) (His tag)
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- Target See all CD73 (NT5E) Proteins
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 29-549
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD73 protein is labelled with His tag.
- Purpose
- Mouse CD73/NT5E Protein
- Sequence
- Trp29-Lys549
- Characteristics
- Recombinant Mouse CD73/NT5E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp29-Lys549.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Mouse CD73, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 31ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in 20 mM Tris, 120 mM NaCl ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
- Alternative Name
- CD73 (NT5E Products)
- Synonyms
- CALJA Protein, CD73 Protein, E5NT Protein, NT Protein, NT5 Protein, NTE Protein, eN Protein, eNT Protein, zgc:63784 Protein, NT5E Protein, MGC114869 Protein, PSPTO2277 Protein, 2210401F01Rik Protein, AI447961 Protein, Nt5 Protein, 5'-nucleotidase ecto Protein, 5'-nucleotidase, ecto (CD73) Protein, 5'-nucleotidase Protein, 5' nucleotidase, ecto Protein, NT5E Protein, nt5e Protein, nt5e.S Protein, PSPTO_2277 Protein, CBU_1233 Protein, MCA1322 Protein, Nt5e Protein
- Background
- CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside-5'-monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury.
- Molecular Weight
- 58.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
- UniProt
- Q61503
- Pathways
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
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