CLEC1A Protein (AA 74-280) (Fc Tag)

Catalog No. ABIN7274352

Product Details

Target: CLEC1A (C-Type Lectin Domain Family 1, Member A (CLEC1A))

Protein Type: Recombinant

Protein Characteristics: AA 74-280

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CLEC1A protein is labelled with Fc Tag.

Purpose: Human CLEC-1 Protein

Sequence: Gln74-Asp280

Characteristics: Recombinant Human CLEC-1 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Gln74-Asp280.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CLEC-1, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC-1 Antibody, hFc Tag with the EC50 22.1ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CLEC1A (C-Type Lectin Domain Family 1, Member A (CLEC1A))

Alternative Name: CLEC-1 (CLEC1A Products)

Synonyms: RGD1566110 Protein, CLEC1 Protein, 5930406N14Rik Protein, C-type lectin domain family 1, member A Protein, C-type lectin domain family 1 member A Protein, C-type lectin domain family 1, member a Protein, Clec1a Protein, CLEC1A Protein

Background: C-type lectin-like receptor-1 (CLEC-1) is a member of the Dectin-1 cluster of pattern recognition receptors (PRRs). It is involved in host immunity, has immunoregulatory function, and supports allograft tolerance. CLEC-1 may act as a negative regulator of Dectin-1 induced host inflammatory response via suppressing neutrophils recruitment and production of pro-inflammatory cytokine IL-1β production in response to A. fumigatus keratitis.

Molecular Weight: 51.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

UniProt: Q8NC01