DKK1 Protein (AA 32-142) (Fc-Avi Tag)

Catalog No. ABIN7274472

Product Details

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Protein Type: Recombinant

Protein Characteristics: AA 32-142

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This DKK1 protein is labelled with Fc-Avi Tag.

Purpose: Human DKK1 N terminal Domain Protein

Sequence: Thr32-Asp142

Characteristics: Recombinant Human DKK1 N terminal Domain Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Thr32-Asp142.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human DKK1 N terminal Domain, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 2.8μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Alternative Name: DKK1 (DKK1 Products)

Synonyms: DKK-1 Protein, SK Protein, AI414495 Protein, SGY Protein, SGY-1 Protein, SGY1 Protein, Soggy Protein, mSgy Protein, dkk1 Protein, dkk Protein, dkk-1 Protein, xdkk1 Protein, Xdkk-1 Protein, Dickkopf1 Protein, dickkopf-1 Protein, mdkk-1 Protein, dkk1-A Protein, dickkopf WNT signaling pathway inhibitor 1 Protein, dickkopf like acrosomal protein 1 Protein, dickkopf-related protein 1 Protein, dickkopf-like 1 Protein, dickkopf WNT signaling pathway inhibitor 1b Protein, dickkopf protein Protein, dickkopf WNT signaling pathway inhibitor 1 S homeolog Protein, DKK1 Protein, Dkk1 Protein, DKKL1 Protein, LOC100228789 Protein, Dkkl1 Protein, dkk1b Protein, dkk1 Protein, dickkopf Protein, dkk1.S Protein

Background: Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.

Molecular Weight: 40.25 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

UniProt: O94907

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation