TNFRSF10B Protein (AA 56-182) (Fc Tag)

Catalog No. ABIN7274496

Product Details

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Protein Type: Recombinant

Protein Characteristics: AA 56-182

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This TNFRSF10B protein is labelled with Fc Tag.

Purpose: Human TRAIL R2/DR5/TNFRSF10B Protein

Sequence: Ile56-Glu182

Characteristics: Recombinant Human TRAIL R2/DR5/TNFRSF10B Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ile56-Glu182.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human TRAIL at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TRAIL R2, hFc Tag with the EC50 of 20ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM Tris, 100 mM NaCl ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Alternative Name: TRAIL R2 (TNFRSF10B Products)

Synonyms: TNFRSF10B Protein, dr5 Protein, cd262 Protein, killer Protein, trick2 Protein, trickb Protein, xDR-M1 Protein, xDR-M2 Protein, ztnfr9 Protein, trailr2 Protein, trick2a Protein, trick2b Protein, CD262 Protein, DR5 Protein, KILLER Protein, KILLER/DR5 Protein, TRAIL-R2 Protein, TRAILR2 Protein, TRICK2 Protein, TRICK2A Protein, TRICK2B Protein, TRICKB Protein, ZTNFR9 Protein, Ly98 Protein, MK Protein, tumor necrosis factor receptor superfamily, member 10b Protein, tumor necrosis factor receptor superfamily member 10b S homeolog Protein, tumor necrosis factor receptor superfamily member 10b L homeolog Protein, tumor necrosis factor receptor superfamily member 10b Protein, TNF receptor superfamily member 10b Protein, Tnfrsf10b Protein, TNFRSF10B Protein, tnfrsf10b.S Protein, tnfrsf10b.L Protein, tnfrsf10b Protein

Background: DR5, also called TRAIL R2, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). DR5 is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis.

Molecular Weight: 41.1 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.

Pathways: p53 Signaling, Apoptosis, Positive Regulation of Endopeptidase Activity