Galectin 3 Protein (LGALS3) (AA 2-250) (His tag)

Catalog No. ABIN7274750

Product Details

Target: Galectin 3 (LGALS3)

Protein Type: Recombinant

Protein Characteristics: AA 2-250

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Galectin 3 protein is labelled with His tag.

Purpose: Human Galectin 3 Protein

Sequence: Ala2-Ile250

Characteristics: Recombinant Human Galectin 3 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ala2-Ile250.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Galectin 3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM HEPES, 150 mM NaCl, 5 mM EDTA, 2 mM TCEP ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Preservative: Other preservative

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Galectin 3 (LGALS3)

Alternative Name: Galectin 3 (LGALS3 Products)

Synonyms: CBP 35 Protein, Gal-3 Protein, Galectin-3 Protein, leg3 Protein, galactoside-binding Protein, galectin-3 Protein, lectin Protein, soluble Protein, CBP35 Protein, GAL3 Protein, GALBP Protein, GALIG Protein, L31 Protein, LGALS2 Protein, MAC2 Protein, MGC52947 Protein, MGC75803 Protein, LGALS3 Protein, gal-3 Protein, GBP Protein, L-34 Protein, Mac-2 Protein, gal3 Protein, galectin 3 Protein, Galectin-3 Protein, avian beta-defensin 3 Protein, lectin, galactoside-binding, soluble, 3 L homeolog Protein, lectin, galactoside-binding, soluble, 3 Protein, galectin 2 Protein, lectin, galactose binding, soluble 3 Protein, LGALS3 Protein, leg3 Protein, AvBD3 Protein, lgals3.L Protein, lgals3 Protein, LGALS2 Protein, Lgals3 Protein

Target Type: Chemical

Background: Human Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain.Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity).

Molecular Weight: 27.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.

UniProt: P17931

Pathways: RTK Signaling