Osteoactivin Protein (GPNMB) (AA 23-502) (His tag)
Quick Overview for Osteoactivin Protein (GPNMB) (AA 23-502) (His tag) (ABIN7274754)
Target
See all Osteoactivin (GPNMB) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 23-502
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Purification tag / Conjugate
- This Osteoactivin protein is labelled with His tag.
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Purpose
- Mouse Osteoactivin/GPNMB Protein
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Sequence
- Lys23-Asn502
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Characteristics
- Recombinant Mouse Osteoactivin/GPNMB Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys23-Asn502.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per ug by the LAL method.
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Biological Activity Comment
- Mouse Osteoactivin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse Osteoactivin is greater than 95% as determined by SEC-HPLC.
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Osteoactivin (GPNMB) (AA 23-486) (Active) protein (His tag)
GPNMB Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Osteoactivin (GPNMB) (AA 23-496) protein (His tag)GPNMB Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Osteoactivin (GPNMB) (AA 1-572) protein (GST tag)GPNMB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Osteoactivin (GPNMB) (AA 22-474) protein (His tag)GPNMB Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
Osteoactivin (GPNMB) (AA 22-474) protein (His tag)GPNMB Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % SDS
Osteoactivin (GPNMB) protein (His tag)GPNMB Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
Osteoactivin (GPNMB) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)GPNMB Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- Osteoactivin (GPNMB)
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Alternative Name
- Osteoactivin
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Background
- GPNMB is highly expressed in macrophages and microglia, which are cells involved with innate immune response in the periphery and the brain, respectively. Some studies have shown increased levels of GPNMB in pro-inflammatory conditions, such as LPS treatment, and in pathological conditions, such as neurodegenerative diseases and cancer.
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Molecular Weight
- 54.7 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
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NCBI Accession
- NP_444340
Target
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