GP6 Protein (AA 21-267) (His tag)
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- Target See all GP6 Proteins
- GP6 (Glycoprotein VI (Platelet) (GP6))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 21-267
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This GP6 protein is labelled with His tag.
- Purpose
- Human GPVI Protein
- Sequence
- Gln21-Lys267
- Characteristics
- Recombinant Human GPVI Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln21-Lys267.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human GPVI, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GPVI Antibody, hFc Tag with the EC50 of 14.8ng/ml determined by ELISA. See testing image for detail.
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Glycoprotein VI (Platelet) (GP6) (AA 21-251) protein (His tag)
GP6 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Glycoprotein VI (Platelet) (GP6) (AA 22-265) protein (His tag)GP6 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Glycoprotein VI (Platelet) (GP6) (AA 21-267) protein (His tag)Crystallography grade GP6 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Glycoprotein VI (Platelet) (GP6) (AA 22-265) protein (His tag)Crystallography grade GP6 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Glycoprotein VI (Platelet) (GP6) (AA 21-339) protein (rho-1D4 tag)Crystallography grade GP6 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Glycoprotein VI (Platelet) (GP6) (AA 22-313) protein (rho-1D4 tag)Crystallography grade GP6 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- GP6 (Glycoprotein VI (Platelet) (GP6))
- Alternative Name
- GPVI (GP6 Products)
- Synonyms
- BDPLT11 Protein, GPIV Protein, GPVI Protein, 9830166G18Rik Protein, Gm469 Protein, Gpvi Protein, GP6 Protein, glycoprotein VI platelet Protein, glycoprotein 6 (platelet) Protein, glycoprotein VI Protein, platelet glycoprotein VI Protein, GP6 Protein, Gp6 Protein, LOC103346235 Protein
- Background
- Although platelets are best known for their role in hemostasis, they are also crucial in development, host defense, inflammation, and tissue repair. Many of these roles are regulated by the immune-like receptors glycoprotein VI (GPVI) and C-type lectin receptor 2 (CLEC-2), which signal through an immunoreceptor tyrosine-based activation motif (ITAM). GPVI is activated by collagen in the subendothelial matrix, by fibrin and fibrinogen in the thrombus, and by a remarkable number of other ligands.
- Molecular Weight
- 28 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
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