TNFRSF18 Protein (AA 26-161) (His tag)

Catalog No. ABIN7274786

Recombinant TNFRSF18 protein expressed in HEK-293 Cells.

Quick Overview for TNFRSF18 Protein (AA 26-161) (His tag) (ABIN7274786)

Target: TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-161

Purification tag / Conjugate: This TNFRSF18 protein is labelled with His tag.

Purpose: Human GITR/TNFRSF18 Protein

Sequence: Gln26-Glu161

Characteristics: Recombinant Human GITR/TNFRSF18 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln26-Glu161.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human GITR, His Tag captured on CM5 Chip via Anti-his antibody can bind Human GITR Ligand Trimer, hFc Tag with an affinity constant of 4.47 nM as determined in SPR assay (Biacore T200).,Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 2.60ug/ml determined by ELISA (QC Test).,Immobilized Human GITR, His Tag at 0.5ug (100ul/Well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA.,Human GITR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%,The purity of Human GITR is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))

Alternative Name: GITR

Background: GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

Molecular Weight: 15.4 kDa. Due to glycosylation, the protein migrates to 25-28 kDa based on Bis-Tris PAGE result.

UniProt: Q9Y5U5

Pathways: Cancer Immune Checkpoints