ERBB4 Protein (AA 26-651) (His-Avi Tag)
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- Target See all ERBB4 Proteins
- ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 26-651
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ERBB4 protein is labelled with His-Avi Tag.
- Purpose
- Human Her4/ErbB4 Protein
- Sequence
- Gln26-Pro651
- Characteristics
- Recombinant Human Her4/ErbB4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln26-Pro651.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Her4 at 1μg/ml (100μl/well) on the plate. Dose response curve for Human NRG1 Beta 1, hFc Tag with the EC50 of 34.7ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))
- Alternative Name
- Her4 (ERBB4 Products)
- Synonyms
- erbb4 Protein, si:ch211-227n20.1 Protein, Her4 Protein, c-erbB-4 Protein, HER4 Protein, p180erbB4 Protein, erb-b2 receptor tyrosine kinase 4 Protein, erb-b2 receptor tyrosine kinase 4a Protein, v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) Protein, erb-b2 receptor tyrosine kinase 4 L homeolog Protein, receptor tyrosine-protein kinase ErbB-4 Protein, ERBB4 Protein, erbb4a Protein, erbb4 Protein, erbb4.L Protein, Tsp_13582 Protein, Erbb4 Protein
- Background
- Her4, a member of the EGF receptor family, plays a variety of roles in physiological and pathological states. Genetic studies have indicated a link between Her4 and type 2 diabetes and obesity. Her4 may play an important role in glucose homeostasis and lipogenesis. Her4 deficiency-related obesity and adipose tissue inflammation may contribute to the development of MetS.
- Molecular Weight
- 72.7 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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