IL27 Receptor alpha Protein (AA 34-516) (Fc Tag)
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- Target See all IL27 Receptor alpha (IL27RA) Proteins
- IL27 Receptor alpha (IL27RA) (Interleukin 27 Receptor, alpha (IL27RA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 34-516
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IL27 Receptor alpha protein is labelled with Fc Tag.
- Purpose
- Human IL-27RA/TCCR Protein
- Sequence
- Gly34-Lys516
- Characteristics
- Recombinant Human IL-27RA/TCCR Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly34-Lys516.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 36.1 determined by ELISA. The affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Interleukin 27 Receptor, alpha (IL27RA) (AA 34-516) protein (His tag)
IL27RA Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Interleukin 27 Receptor, alpha (IL27RA) (AA 33-132) protein (GST tag)IL27RA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 27 Receptor, alpha (IL27RA) (AA 33-516) protein (His tag)Crystallography grade IL27RA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Interleukin 27 Receptor, alpha (IL27RA) (AA 25-510) protein (His tag)Crystallography grade IL27RA Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Interleukin 27 Receptor, alpha (IL27RA) (AA 33-636) protein (rho-1D4 tag)Crystallography grade IL27RA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Interleukin 27 Receptor, alpha (IL27RA) (AA 25-623) protein (rho-1D4 tag)Crystallography grade IL27RA Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS, 5 mM DTT ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IL27 Receptor alpha (IL27RA) (Interleukin 27 Receptor, alpha (IL27RA))
- Alternative Name
- IL-27 Ra (IL27RA Products)
- Synonyms
- IL27RA Protein, CRL1 Protein, IL-27RA Protein, IL27R Protein, TCCR Protein, WSX1 Protein, zcytor1 Protein, IL-27R Protein, Tccr Protein, WSX-1 Protein, Wsx1 Protein, interleukin 27 receptor subunit alpha Protein, interleukin-27 receptor subunit alpha Protein, interleukin 27 receptor, alpha Protein, IL27RA Protein, LOC484907 Protein, Il27ra Protein
- Background
- A multicenter, web-based Thyroid Cancer and Tumor Collaborative Registry (TCCR, http://tccr.unmc.edu) allows for the collection and management of various data on thyroid cancer (TC) and thyroid nodule (TN) patients. The TCCR is coupled with OpenSpecimen, an open-source biobank management system, to annotate biospecimens obtained from the TCCR subjects.
- Molecular Weight
- 79.3 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result.
- UniProt
- Q6UWB1
- Pathways
- Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response
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