IL-22R alpha 1 & IL-10R beta (AA 16-228) protein (Fc-Avi Tag)
Origin: Human
Host: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Target
- IL-22R alpha 1 & IL-10R beta
- Protein Type
- Recombinant
- Protein Characteristics
- AA 16-228
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- Fc-Avi Tag
- Purpose
- Human IL-22R alpha 1 & IL-10R beta Protein
- Sequence
- His16-Thr228 (IL-22R alpha 1) & Met20-Ser220 (IL-10R beta)
- Specificity
- Uni-Prot: Q8N6P7 (IL-22R alpha 1), Q08334 (IL-10R beta)
- Characteristics
- Recombinant Human IL-22R alpha 1 & IL-10R beta Protein is expressed from HEK293 with hFc tag and Avi Tag at the C-Terminus.It contains His16-Thr228(IL-22R alpha 1) & Met20-Ser220(IL-10R beta).
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-22, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-22R alpha 1&IL-10R beta, hFc Tag with the EC50 of 0.42μg/ml determined by ELISA. See testing image for detail.
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IL-22R alpha 1 & IL-10R beta (AA 16-228) protein (Fc-Avi Tag)
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IL-22R alpha 1 & IL-10R beta (AA 16-228) protein (Fc-Avi Tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IL-22R alpha 1 & IL-10R beta
- Background
- IL-22Rα1 & IL-10Rβ are receptors for IL-22 and IL-10, mediate the activation of JAK-STAT, MAPK and other signaling pathways, and participate in the process of many inflammatory diseases. IL--22 Rα1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 Rβ or IL-20 Rβ and the respective ligands is required for signal transduction.
- Molecular Weight
- 52.07 kDa(IL-22R alpha 1)&48.85 kDa(IL-10R beta). Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.
- UniProt
- Q8N6P7
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