ITGAV/ITGB6 Protein (AA 31-993) (His tag)
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- Target See all ITGAV/ITGB6 products
- ITGAV/ITGB6 (Integrin AlphaV Beta6 (ITGAV/ITGB6))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 31-993
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Origin
- Rhesus Monkey
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ITGAV/ITGB6 protein is labelled with His tag.
- Purpose
- Rhesus macaque Integrin alpha V beta 6 (ITGAV & ITGB6) Heterodimer Protein
- Sequence
- Phe31-Pro993 (ITGAV) acidic tail & Gly22-Asn707 (ITGB6) basic tail
- Specificity
- Uni-Prot: F6QSK2 (ITGAV), A0A1D5QZT0 (ITGB6)
- Characteristics
- Recombinant Rhesus macaque Integrin alpha V beta 6 (ITGAV & ITGB6) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe31-Pro993(ITGAV) acidic tail & Gly22-Asn707(ITGB6) basic tail.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Rhesus macaque ITGAV&ITGB6, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-alpha-V Antibody, hFc Tag with the EC50 of 32.4ng/ml determined by ELISA. See testing image for detail.
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Integrin AlphaV Beta6 (ITGAV/ITGB6) (AA 31-988) protein (His tag)
ITGAV/ITGB6 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Integrin AlphaV Beta6 (ITGAV/ITGB6) (AA 31-992) (Active) protein (His tag)ITGAV/ITGB6 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Integrin AlphaV Beta6 (ITGAV/ITGB6) (AA 31-992) (Active) protein (His tag,AVI tag,Biotin)ITGAV/ITGB6 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by reduced SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- ITGAV/ITGB6 (Integrin AlphaV Beta6 (ITGAV/ITGB6))
- Alternative Name
- Integrin alpha V beta 6 (ITGAV & ITGB6) Heterodimer (ITGAV/ITGB6 Products)
- Background
- ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. ITGAV&ITGB6 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1.
- Molecular Weight
- 112.1 kDa (ITGAV)&79 kDa (ITGB6). Due to glycosylation, the protein migrates to 90-140 kDa based on Tris-Bis PAGE result.
- UniProt
- F6QSK2
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