ITGAV/ITGB6 Protein (AA 31-992) (His tag,AVI tag,Biotin)

Catalog No. ABIN5674599

Product Details

Target: ITGAV/ITGB6 (Integrin AlphaV Beta6 (ITGAV/ITGB6))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 31-992

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ITGAV/ITGB6 protein is labelled with His tag,AVI tag,Biotin.

Brand: PrecisionAvi

Sequence: AA 31-992

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Characteristics: Biotinylated Human ITGAV & ITGB6 Heterodimer Protein, produced by co-expression of ITGAV and ITGB6, has a calculated MW of 113 kDa (ITGAV) and 79.6 kDa (ITGB6). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine

Purity: >90 % as determined by reduced SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris, 150 mM NaCl, pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: ITGAV/ITGB6 (Integrin AlphaV Beta6 (ITGAV/ITGB6))

Alternative Name: Integrin alpha V beta 6 (ITGAV/ITGB6 Products)

Background: Integrin alpha V beta 6 is a heterodimer of beta-6 associating with alpha-V. Integrin alpha-V beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. Also, Integrin alpha-V beta-6 acts as a receptor for coxsackievirus A9 and coxsackievirus B1 as well as herpes simplex virus-1/HHV-1. Furthermore, it binds the TGF-beta latency-associated peptide (LAP) and activates TGF-beta 1 or TGF-beta 3 from large latent complexes. This activation requires interaction with LTBP-1 and fibronectin, and is enhanced by PAR-1.

Molecular Weight: 114.7 kDa (ITGAV) & 79.6 kDa (ITGB6)