Klotho beta Protein (KLB) (His tag)
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- Target See all Klotho beta (KLB) Proteins
- Klotho beta (KLB)
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Klotho beta protein is labelled with His tag.
- Sequence
- Met30-Thr983
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Beta Klotho at 2μg/ml (100μl/Well). Dose response curve for Anti-Beta Klotho Antibody1, hFc Tag with the EC50 of 38.5ng/ml determined by ELISA. The affinity constant of 0.54nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Klotho beta (KLB) (AA 53-994) protein (His tag)
KLB Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Klotho beta (KLB) (AA 1-1044) protein (Strep Tag)Crystallography grade KLB Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Klotho beta (KLB) (AA 1-1043) protein (Strep Tag)Crystallography grade KLB Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Klotho beta (KLB) (AA 1-996) protein (His tag)Crystallography grade KLB Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Klotho beta (KLB) (AA 1-994) protein (His tag)Crystallography grade KLB Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Klotho beta (KLB)
- Alternative Name
- Beta Klotho (KLB Products)
- Synonyms
- KLB Protein, BKL Protein, AV071179 Protein, betaKlotho Protein, Klb-ps1 Protein, RGD1308227 Protein, klotho beta Protein, KLB Protein, Klb Protein
- Background
- BetaKlotho, beta-klotho, BKL, KLB, klotho beta like, Klotho beta, MGC142213,Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Klb is prominent within the hypothalamus, which is consistent with its metabolic functions, but diverse roles for Klb are now emerging. Central Klb expression is low but discrete and may govern FGF-targeted sites.
- Molecular Weight
- 111.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.
- UniProt
- Q86Z14
- Pathways
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Growth Factor Binding
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