LRG1 Protein (AA 72-383) (His tag)

Catalog No. ABIN7275201

Product Details

Target: LRG1 (Leucine-Rich alpha-2 Glycoprotein 1 (LRG1))

Protein Type: Recombinant

Protein Characteristics: AA 72-383

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This LRG1 protein is labelled with His tag.

Purpose: Cynomolgus LRG1 Protein

Sequence: Val72-Gln383

Characteristics: Recombinant Cynomolgus LRG1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val72-Gln383.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus LRG1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-LRG1 Antibody, hFc Tag with the EC50 of 0.34μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LRG1 (Leucine-Rich alpha-2 Glycoprotein 1 (LRG1))

Alternative Name: LRG1 (LRG1 Products)

Synonyms: lrg Protein, HMFT1766 Protein, LRG Protein, 1300008B03Rik Protein, 2310031E04Rik Protein, Lrg Protein, Lrhg Protein, si:dkey-90m5.4 Protein, leucine rich alpha-2-glycoprotein 1 Protein, leucine-rich alpha-2-glycoprotein 1 L homeolog Protein, leucine-rich alpha-2-glycoprotein 1 Protein, perilipin-5 Protein, si:dkey-90m5.4 Protein, LRG1 Protein, lrg1.L Protein, Lrg1 Protein, PLIN5 Protein

Background: Diabetic nephropathy (DN) is an important public health concern of increasing proportions and the leading cause of end-stage renal disease (ESRD) in diabetic patients. It is one of the most common long-term microvascular complications of diabetes mellitus that is characterized by proteinuria and glomerular structural changes. LRG1 is a novel pro-angiogenic factors involved in the abnormal angiogenesis and renal fibrosis in DN.

Molecular Weight: 35.69 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

UniProt: A0A2K5VVA4

Pathways: Brown Fat Cell Differentiation