LRG1 Protein (AA 36-347) (His tag)
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- Target See all LRG1 Proteins
- LRG1 (Leucine-Rich alpha-2 Glycoprotein 1 (LRG1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 36-347
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This LRG1 protein is labelled with His tag.
- Purpose
- Human LRG1 Protein
- Sequence
- Val36-Gln347
- Characteristics
- Recombinant Human LRG1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val36-Gln347.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human LRG1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LRG1 Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA. See testing image for detail.
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Leucine-Rich alpha-2 Glycoprotein 1 (LRG1) (AA 72-383) protein (His tag)
LRG1 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leucine-Rich alpha-2 Glycoprotein 1 (LRG1) (AA 33-342) protein (His tag)LRG1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leucine-Rich alpha-2 Glycoprotein 1 (LRG1) (AA 37-347) protein (GST tag)LRG1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Leucine-Rich alpha-2 Glycoprotein 1 (LRG1) (AA 36-347) protein (His tag)LRG1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- LRG1 (Leucine-Rich alpha-2 Glycoprotein 1 (LRG1))
- Alternative Name
- LRG1 (LRG1 Products)
- Synonyms
- lrg Protein, HMFT1766 Protein, LRG Protein, 1300008B03Rik Protein, 2310031E04Rik Protein, Lrg Protein, Lrhg Protein, si:dkey-90m5.4 Protein, leucine rich alpha-2-glycoprotein 1 Protein, leucine-rich alpha-2-glycoprotein 1 L homeolog Protein, leucine-rich alpha-2-glycoprotein 1 Protein, perilipin-5 Protein, si:dkey-90m5.4 Protein, LRG1 Protein, lrg1.L Protein, Lrg1 Protein, PLIN5 Protein
- Background
- Diabetic nephropathy (DN) is an important public health concern of increasing proportions and the leading cause of end-stage renal disease (ESRD) in diabetic patients. It is one of the most common long-term microvascular complications of diabetes mellitus that is characterized by proteinuria and glomerular structural changes. LRG1 is a novel pro-angiogenic factors involved in the abnormal angiogenesis and renal fibrosis in DN.
- Molecular Weight
- 35.4 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Tris-Bis PAGE result.
- UniProt
- P02750
- Pathways
- Brown Fat Cell Differentiation
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