Lrig1 Protein (AA 35-779) (His tag)

Catalog No. ABIN7275205

Product Details

Target: Lrig1 (Leucine-Rich Repeats and Immunoglobulin-Like Domains 1 (Lrig1))

Protein Type: Recombinant

Protein Characteristics: AA 35-779

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Lrig1 protein is labelled with His tag.

Purpose: Human LRIG1 Protein

Sequence: Ala35-Ser779

Characteristics: Recombinant Human LRIG1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala35-Ser779.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human LRIG1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-LRIG1 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 50 mM MES, 150 mM NaCl, 1 mM EDTA ( pH 5.0).

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM MES, 150 mM NaCl, 1 mM EDTA ( pH 5.0). Normally 8 % trehalose is added as protectant before lyophilization.

Preservative: Other preservative

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Lrig1 (Leucine-Rich Repeats and Immunoglobulin-Like Domains 1 (Lrig1))

Alternative Name: LRIG1 (Lrig1 Products)

Synonyms: LIG-1 Protein, LIG1 Protein, D6Bwg0781e Protein, Img Protein, leucine rich repeats and immunoglobulin like domains 1 Protein, leucine-rich repeats and immunoglobulin-like domains 1 Protein, LRIG1 Protein, Lrig1 Protein

Background: The leucine-rich repeats and immunoglobulin-like domains (LRIG)-1 is a tumor suppressor gene that belongs to the LRIG family. LRIG1 expression has prognostic significance in various human cancers. Somatic mutations, which are associated with a certain rate of response to targeted therapies, are ubiquitously found in human non-small cell lung cancer (NSCLC). LRIG1 was an independent prognostic factor for OS of NSCLC patients. LRIG1 in combination with other clinicopathological risk factors was a stronger prognostic model than clinicopathological risk factors alone.

Molecular Weight: 83 kDa. Due to glycosylation, the protein migrates to 85-105 kDa based on Tris-Bis PAGE result.