MANSC1 Protein (AA 27-385) (His tag)
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- Target See all MANSC1 Proteins
- MANSC1 (MANSC Domain Containing 1 (MANSC1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-385
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MANSC1 protein is labelled with His tag.
- Purpose
- Human MANSC1 Protein
- Sequence
- Gln27-Leu385
- Characteristics
- Recombinant Human MANSC1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln27-Leu385.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human MANSC1, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-MANSC1 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- MANSC1 (MANSC Domain Containing 1 (MANSC1))
- Alternative Name
- MANSC1 (MANSC1 Products)
- Synonyms
- MANSC1 Protein, DKFZp469A172 Protein, 9130403P13Rik Protein, LOH12CR3 Protein, MANSC domain containing 1 Protein, MANSC1 Protein, Mansc1 Protein, LOC777123 Protein
- Background
- MANSC1 contains 1 MANSC domain. MANSC is a seven-cysteine-containing domain present in animal membrane and extracellular proteins. MANSC (motif at N terminus with seven cysteines) is a novel domain with a well-conserved seven-cysteine motif that is present at the N terminus of membrane and extracellular proteins, including low-density lipoprotein receptor-related protein 11 (LRP-11), hepatocyte growth factor activator inhibitor 1 (HAI-1) and some uncharacterized proteins encoded by multicellular animals from Mollusca to Chordata. The MANSC domain in HAI-2 might function through binding with hepatocyte growth factor activator and matriptase[1].
- Molecular Weight
- 39.9 kDa. Due to glycosylation, the protein migrates to 113-116 kDa based on Tris-Bis PAGE result.
- UniProt
- Q9H8J5
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