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MANSC1 Protein (AA 27-385) (His tag)

MANSC1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Catalog No. ABIN7275227
  • Target See all MANSC1 Proteins
    MANSC1 (MANSC Domain Containing 1 (MANSC1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 27-385
    Origin
    • 3
    • 3
    • 1
    Human
    Source
    • 4
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This MANSC1 protein is labelled with His tag.
    Purpose
    Human MANSC1 Protein
    Sequence
    Gln27-Leu385
    Characteristics
    Recombinant Human MANSC1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln27-Leu385.
    Purity
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human MANSC1, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-MANSC1 Antibody, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA. See testing image for detail.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    Storage
    -20 °C,-80 °C
    Storage Comment
    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    MANSC1 (MANSC Domain Containing 1 (MANSC1))
    Alternative Name
    MANSC1 (MANSC1 Products)
    Synonyms
    MANSC1 Protein, DKFZp469A172 Protein, 9130403P13Rik Protein, LOH12CR3 Protein, MANSC domain containing 1 Protein, MANSC1 Protein, Mansc1 Protein, LOC777123 Protein
    Background
    MANSC1 contains 1 MANSC domain. MANSC is a seven-cysteine-containing domain present in animal membrane and extracellular proteins. MANSC (motif at N terminus with seven cysteines) is a novel domain with a well-conserved seven-cysteine motif that is present at the N terminus of membrane and extracellular proteins, including low-density lipoprotein receptor-related protein 11 (LRP-11), hepatocyte growth factor activator inhibitor 1 (HAI-1) and some uncharacterized proteins encoded by multicellular animals from Mollusca to Chordata. The MANSC domain in HAI-2 might function through binding with hepatocyte growth factor activator and matriptase[1].
    Molecular Weight
    39.9 kDa. Due to glycosylation, the protein migrates to 113-116 kDa based on Tris-Bis PAGE result.
    UniProt
    Q9H8J5
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