MICA Protein (His-Avi Tag,Biotin)
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- Target See all MICA Proteins
- MICA (MHC Class I Polypeptide-Related Sequence A (MICA))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MICA protein is labelled with His-Avi Tag,Biotin.
- Sequence
- Glu24-Gln308
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human NKG2D, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human MICA, His Tag with the EC50 of 0.32μg/ml determined by ELISA. See testing image for detail.
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MHC Class I Polypeptide-Related Sequence A (MICA) (AA 24-308) protein (His-Avi Tag)
MICA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
MHC Class I Polypeptide-Related Sequence A (MICA) (AA 1-284) protein (His tag)MICA Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
MHC Class I Polypeptide-Related Sequence A (MICA) protein (His tag)MICA Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
MHC Class I Polypeptide-Related Sequence A (MICA) (AA 24-308) (Active) protein (Fc Tag)MICA Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
MHC Class I Polypeptide-Related Sequence A (MICA) (AA 24-306) protein (His tag)MICA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
MHC Class I Polypeptide-Related Sequence A (MICA) (AA 24-307) protein (His tag)MICA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
MHC Class I Polypeptide-Related Sequence A (MICA) (AA 1-383) protein (GST tag)MICA Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
MHC Class I Polypeptide-Related Sequence A (MICA) (AA 24-307) protein (His tag)Crystallography grade MICA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
MHC Class I Polypeptide-Related Sequence A (MICA) (AA 24-383) protein (rho-1D4 tag)Crystallography grade MICA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- MICA (MHC Class I Polypeptide-Related Sequence A (MICA))
- Alternative Name
- MICA (MICA Products)
- Synonyms
- MIC-A Protein, PERB11.1 Protein, MHC class I polypeptide-related sequence A Protein, MICA Protein
- Background
- FLJ60820, MGC111087, MICA, PERB11.1, DAMA-345G11.2, FLJ36918, FLJ60820, MGC21250,TMICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85 % amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin.
- Molecular Weight
- 35.8 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result.
- UniProt
- Q96QC4
- Pathways
- Activation of Innate immune Response, Transition Metal Ion Homeostasis
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