KLRC1 Protein (AA 100-233) (His-Avi Tag,Biotin)
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- Target See all KLRC1 Proteins
- KLRC1 (Killer Cell Lectin-Like Receptor Subfamily C, Member 1 (KLRC1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 100-233
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This KLRC1 protein is labelled with His-Avi Tag,Biotin.
- Purpose
- Biotinylated Human NKG2A/CD159a Protein
- Sequence
- Arg100-Leu233
- Characteristics
- Recombinant Biotinylated Human NKG2A/CD159a Protein is expressed from HEK293 with His tag and Avi tag at N-Terminus.It contains Arg100-Leu233.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-NKG2A Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human NKG2A, His Tag with the EC50 of 0.21μg/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- KLRC1 (Killer Cell Lectin-Like Receptor Subfamily C, Member 1 (KLRC1))
- Alternative Name
- NKG2A (KLRC1 Products)
- Synonyms
- rNKG2A Protein, NKG2-A Protein, NKG2A Protein, KLRC1 Protein, CD159A Protein, NKG2 Protein, CD159a Protein, NKG2B Protein, killer cell lectin like receptor C1 Protein, NKG2-A/NKG2-B type II integral membrane protein-like Protein, killer cell lectin-like receptor subfamily C, member 3 Protein, killer cell lectin-like receptor subfamily C, member 1 Protein, Klrc1 Protein, KLRC1 Protein, NKG2A Protein, KLRC3 Protein
- Background
- NKG2, also known as NKG2A(CD159A), is a member of the killer cell lectin-like receptor family. NKG2A marks a unique immune effector subset preferentially co-expressing the tissue-resident CD103 Molecule, but not immune checkpoint inhibitors.It is expressed only in NK-cells, but not in T-cells or B-cells.
- Molecular Weight
- 18.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
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