NOTCH3 Protein (AA 1378-1640) (His tag)

Catalog No. ABIN7275361

Product Details

Target: NOTCH3 (Notch 3 (NOTCH3))

Protein Type: Recombinant

Protein Characteristics: AA 1378-1640

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This NOTCH3 protein is labelled with His tag.

Purpose: Human Notch 3 Protein

Sequence: Ala1378-Ser1640

Characteristics: Recombinant Human Notch 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala1378-Ser1640.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Notch 3, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: NOTCH3 (Notch 3 (NOTCH3))

Alternative Name: Notch 3 (NOTCH3 Products)

Synonyms: CADASIL Protein, CASIL Protein, IMF2 Protein, AW229011 Protein, N3 Protein, hpbk Protein, fa14b08 Protein, notch5 Protein, wu:fa14b08 Protein, notch 3 Protein, NOTCH3 Protein, Notch3 Protein, notch3 Protein

Background: Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes. The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Molecular Weight: 30.18 kDa. Due to furin-like protease cleavage and glycosylation, the protein migrates to 27-30 kDa and 10-12 kDa based on Tris-Bis PAGE result.

UniProt: Q9UM47

Pathways: Notch Signaling