RGMA Protein (His-Avi Tag)
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- Target See all RGMA Proteins
- RGMA (RGM Domain Family, Member A (RGMA))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This RGMA protein is labelled with His-Avi Tag.
- Sequence
- Cys48-Gly422
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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RGM Domain Family, Member A (RGMA) (AA 48-422) protein (His-Avi Tag,Biotin)
RGMA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
RGM Domain Family, Member A (RGMA) (AA 169-422) protein (His tag)RGMA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
RGM Domain Family, Member A (RGMA) (AA 326-423) protein (GST tag)RGMA Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
RGM Domain Family, Member A (RGMA) (AA 169-424) protein (His tag)Crystallography grade RGMA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
RGM Domain Family, Member A (RGMA) (AA 170-427) protein (His tag)Crystallography grade RGMA Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
RGM Domain Family, Member A (RGMA) protein (Myc-DYKDDDDK Tag)RGMA Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- RGMA (RGM Domain Family, Member A (RGMA))
- Alternative Name
- RGMa (RGMA Products)
- Synonyms
- RGM Protein, BC059072 Protein, C230063O06 Protein, repulsive guidance molecule family member a Protein, repulsive guidance molecule family member A Protein, RGMA Protein, Rgma Protein
- Background
- RGMA, RGM,Repulsive guidance molecule (RGM) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that has diverse functions in the developing and pathological central nervous system (CNS). The binding of RGM to its receptor neogenin regulates axon guidance, neuronal differentiation, and survival during the development of the CNS. RGMa induces T cell activation in experimental autoimmune encephalomyelitis (EAE), which is the animal model of multiple sclerosis (MS). RGM is expressed in pathogenic Th17 cells and induces neurodegeneration by binding to neogenin.
- Molecular Weight
- 44.5 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result.
- Pathways
- Tube Formation
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