ROBO4 Protein (AA 27-469) (His tag)
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- Target See all ROBO4 Proteins
- ROBO4 (Roundabout, Axon Guidance Receptor, Homolog 4 (ROBO4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-469
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ROBO4 protein is labelled with His tag.
- Purpose
- Human ROBO4 Protein
- Sequence
- Ala27-Glu469
- Characteristics
- Recombinant Human ROBO4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala27-Glu469.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human ROBO4, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 26.1ng/ml determined by ELISA. See testing image for detail.
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Roundabout, Axon Guidance Receptor, Homolog 4 (ROBO4) (AA 28-480) protein (His tag)
ROBO4 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Roundabout, Axon Guidance Receptor, Homolog 4 (ROBO4) (AA 28-235) protein (His tag)ROBO4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Roundabout, Axon Guidance Receptor, Homolog 4 (ROBO4) (AA 28-1007) protein (His tag)Crystallography grade ROBO4 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Roundabout, Axon Guidance Receptor, Homolog 4 (ROBO4) (AA 28-1012) protein (His tag)Crystallography grade ROBO4 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- ROBO4 (Roundabout, Axon Guidance Receptor, Homolog 4 (ROBO4))
- Alternative Name
- ROBO4 (ROBO4 Products)
- Synonyms
- ECSM4 Protein, MRB Protein, 1200012D01Rik Protein, AI593217 Protein, sc:d0823 Protein, wu:fa16f09 Protein, roundabout guidance receptor 4 Protein, roundabout, axon guidance receptor, homolog 4 (Drosophila) Protein, ROBO4 Protein, Robo4 Protein, robo4 Protein
- Background
- Roundabout4 (Robo4) is a transmembrane receptor that belongs to the Roundabout (Robo) family of axon guidance molecules. Robo4 is an endothelial-specific receptor that participates in endothelial cell migration, proliferation, and angiogenesis and the maintenance of vasculature homeostasis. Robo4 is a promising and potentially valuable therapeutic target for treating pathological angiogenesis and developmental defects in angiogenesis.
- Molecular Weight
- 47.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
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