SEZ6 Protein (AA 20-925) (His tag)
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- Target See all SEZ6 Proteins
- SEZ6 (Seizure Related 6 Homolog (SEZ6))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 20-925
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SEZ6 protein is labelled with His tag.
- Purpose
- Human SEZ6 Protein
- Sequence
- Leu20-His925
- Characteristics
- Recombinant Human SEZ6 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu20-His925.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human SEZ6, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-SEZ6 Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA. See testing image for detail.
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Seizure Related 6 Homolog (SEZ6) (AA 20-927) protein (His tag)
SEZ6 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Seizure Related 6 Homolog (SEZ6) (AA 20-922) protein (His tag)SEZ6 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Seizure Related 6 Homolog (SEZ6) (AA 20-925) protein (His tag)Crystallography grade SEZ6 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Seizure Related 6 Homolog (SEZ6) (AA 20-922) protein (His tag)Crystallography grade SEZ6 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Seizure Related 6 Homolog (SEZ6) (AA 20-994) protein (rho-1D4 tag)Crystallography grade SEZ6 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Seizure Related 6 Homolog (SEZ6) (AA 20-991) protein (rho-1D4 tag)Crystallography grade SEZ6 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Seizure Related 6 Homolog (SEZ6) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SEZ6 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- SEZ6 (Seizure Related 6 Homolog (SEZ6))
- Alternative Name
- SEZ6 (SEZ6 Products)
- Synonyms
- BSRP-C Protein, D11Bhm177e Protein, mKIAA4158 Protein, sez-6 Protein, seizure related gene 6 Protein, seizure related 6 homolog Protein, Sez6 Protein, SEZ6 Protein
- Background
- Seizure-related protein 6 (Sez6) contributes to chronic pain development as sez6 knockout mice show attenuated pain behaviours after peripheral nerve injury, compared with control mice. The type I transmembrane isoform of Sez6 is cleaved by the β-amyloid precursor protein cleavage enzyme 1 (BACE1), resulting in Sez6 extracellular domain shedding from the neuron surface.
- Molecular Weight
- 98.9 kDa. Due to glycosylation, the protein migrates to 145-150 kDa based on Tris-Bis PAGE result.
- Pathways
- Synaptic Membrane
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