SIGLEC10 Protein (Arg119Ala-Mutant) (His-Avi Tag)

Catalog No. ABIN7275604

Product Details

Target: SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))

Protein Type: Recombinant

Protein Characteristics: Arg119Ala-Mutant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SIGLEC10 protein is labelled with His-Avi Tag.

Purpose: Human Siglec-10 (R119A) Protein

Sequence: Met17-Thr546

Characteristics: Recombinant Human Siglec-10(R119A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met17-Thr546.

Purity: > 95 % as determined by Tris-Bis PAGE,

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Siglec-10 (R119A) , His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Siglec-10 (R119A) Antibody, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM Tris,150 mM NaCl, 200 mM Arginine ( pH 8.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))

Alternative Name: Siglec-10 (SIGLEC10 Products)

Synonyms: PRO940 Protein, SIGLEC-10 Protein, SLG2 Protein, Siglecg Protein, SIGLEC10 Protein, slg2 Protein, pro940 Protein, siglec-10 Protein, sialic acid binding Ig like lectin 10 Protein, sialic acid binding Ig-like lectin 10 Protein, sialic acid-binding Ig-like lectin 10 Protein, SIGLEC10 Protein, Siglec10 Protein, LOC484343 Protein, LOC100066644 Protein, siglec10 Protein, LOC100513863 Protein, LOC100340374 Protein

Background: Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.

Molecular Weight: 62.64 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Tris-Bis PAGE result.