Thymic Stromal Lymphopoietin Protein (TSLP) (AA 29-159) (His tag)

Catalog No. ABIN7275799

This Recombinant Thymic Stromal Lymphopoietin protein is expressed in HEK-293 Cells.

Quick Overview for Thymic Stromal Lymphopoietin Protein (TSLP) (AA 29-159) (His tag) (ABIN7275799)

Target: Thymic Stromal Lymphopoietin (TSLP)

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 29-159

Purification tag / Conjugate: This Thymic Stromal Lymphopoietin protein is labelled with His tag.

Purpose: Cynomolgus TSLP Protein

Sequence: Tyr29-Gln159

Characteristics: Recombinant Cynomolgus TSLP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr29-Gln159.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus TSLP, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.,Immobilized Cynomolgus TSLP, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA.,Cynomolgus TSLP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Cynomolgus TSLP, His Tag with an affinity constant of 6.58 nM as determined in BLI assay (Gator® Prime).,Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Cynomolgus TSLP, His Tag with an affinity constant of 2.02 nM as determined in BLI assay (Gator® Prime).,The purity of Cynomolgus TSLP is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Thymic Stromal Lymphopoietin (TSLP)

Alternative Name: TSLP

Background: Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Molecular Weight: 16.3 kDa. Due to furin cleavage site, the protein migrates to 12 kDa&23-30 kDa based on Bis-Tris PAGE result.

UniProt: A0A7N9CAT7